Metagenomic characterization of soil microbial communities in typical chernozem of Moldova

Abstract

Based on the new generation sequencing methods, such as pyrosequencing (Roche/454 Life Sciences), the metagenomic characteristics of typical chernozem microbiomes were determined and described for the first time in Moldova under different land use conditions. It was revealed that the community of prokaryotes is represented by both domains, particularly twelve bacterial phyla (61.4%) and one phylum Archaea (6.2%). Only nine phyla had an abundance above 1.0%: the archaeal phylum Сrenarchaeota (Thaumarchaeota) and the bacterial phyla Proteobacteria, Actinobacteriota, Bacteroidota, Acidobacteriota, Firmicutes, Verrumicrobiota, Platctomycecota, and Myxococcota. Phyla, with a share of less than 1.0%, preferred the conditions of experimental variants of crop rotations. A significant proportion were unidentified microorganisms, the abundance of which varied from 25.8 to 38.2% in spring and from 27.9 to 40.2% in summer. The influence of soil and climatic conditions on the formation of the typical chernozem microbiome was more significant than agricultural practices.